Plant molecular biology practicals

  • Cours (CM) -
  • Cours intégrés (CI) -
  • Travaux dirigés (TD) -
  • Travaux pratiques (TP) 140h
  • Travail étudiant (TE) -

Langue de l'enseignement : Anglais

Description du contenu de l'enseignement

Learning objectives (course content) :
  • Plant hormones and mobilization of starch reserves in germinating grain
  • Plant cell culture and plantlet regeneration
  • Genetic engineering of seed content
  • Induced resistance in wine grape and resveratrol production
  • Transcriptomics and proteomics of stress responses in moss
  • Transient expression of recombinant proteins in plant protoplasts
  • In vivo calcium measurements
  • Microinjection of plant cells
  • Genetics of photoreceptors in plants
  • Transformation of plants by particle bombardment and Agrobacterium infection
  • Genetics of stem cell formation and maintenance in plants
  • Epigenetic imprinting and reciprocal crosses
  • Gene regulation by micro-RNAs
  • Mapping a point mutation in Arabidopsis thaliana

Compétences à acquérir

Objectives in terms of acquired skills :
(ie roughly what the student should be able to understand and apply in practical situations at the end of the course)
  • To familiarize the student with genetics, physiology, genetic engineering and cell biology of plants.
  • To foster scientific communication through seminars, critical review of literature and writing reports of experiments conducted during the course.

Bibliographie, lectures recommandées

References, books, course support :
  • Lab manual of 220 pages.
  • Scientific review and research articles.
  • Lecture, tutorial and discussion periods