Enzymology practicals

  • Cours (CM) 10h
  • Cours intégrés (CI) -
  • Travaux dirigés (TD) 8h
  • Travaux pratiques (TP) 27h
  • Travail étudiant (TE) -

Langue de l'enseignement : Anglais

Description du contenu de l'enseignement

Objectives for knowledge :
  • Understanding and practicing enzymology basics including:
  • Enzymatic activity
  • Kinetics of enzymatic activity
  • Immobilizing enzymes, a lecture and practice at small and medium scales
  • The beta-galactosidase as a prototypic enzyme
  • Hydrolysis of lactose, purified and contained in milk
  • Discussions on the strategies and results

Compétences à acquérir

Objectives in terms of skills :
  • Acquire a good practice for work with proteins including enzymes, with kinetics
  • Using spectrophotometers
  • Acquire the notion of making appropriate graphs, of experimental variability/reproducibility
  • Be able to relate the theory of enzymology to experimental results
  • Be able to analyze and discuss on experimental results
  • Be able to present experimental results

Contact

Responsable

Claire Gaveriaux-Ruff