Objectives in terms of knowledge (course content):

1. CM
   1. The mechanism of translation: what is needed for translation (components and actors), the process, differences in pro- and eukaryotes and what is the product of translation
   2. Post-translation: protein modification, trafficking, gene regulation, quality control
   3. Engineering: IRES, 2A peptides, fluorescent tags
   4. Methods to detect proteins: IHC/IF, purification, WB, IP/Co-IP, ChIP/ChIP-seq, Microscopy and FRET

1. Group work

Students are divided into groups and work on a specific question related to translation process and its regulation. There is a session with the teacher in order to ask questions and guide the groups into the reflection on their subject. Afterwards, they write a little report (5 pages) and prepare a short presentation (10min) to explain their subject to the whole class.

1. TD

Exercises about translation and application. There are 2 sessions: one for BT only and one for CBT only. This allows better participation of the students and give an example of the future examination exercises.
 

Objectives of the lessons :

1. Understanding and acquiring of the mechanisms linked to the dynamics of gene expression in a cell (prokaryotes and eukaryotes) at molecular and cellular levels
2. Knowing some techniques linked to proteins

All documents necessary for the courses and TD are on Moodle.