Course content :

I. Transcription of genetic information in prokaryotes
II. RNA polymerase compounds and function.
III. steps of transcription ; Initiation, transcription elongation and regulation of the transcriptional termination.
IV. Regulation of gene expression: lactose and tryptophan operon
V. Regulation of lambda gene expression.
VI. CRISPR
VII. Biotechnology applications : pETOM, CRISPR etc

 

Objectives in terms of competences:
This course aims at confronting the student with the fundamental principles of molecular biology in prokaryotes. Relevant basic terminology is introduced and general concepts and principles are presented. The differences between pro- and eukaryotes are introduced. Biotechnology applications with regards to transcription and gene regulation are discussed

Bibliography, Course supports :

• Power Point on Moodle
• Biochimie Générale JH Weil 11éme Edition Dunod Sciences
• Genes IX Benjamin Lewin (Jones and Bartlett Publishers, Inc)